Thioredoxin 1 antibody
WebThioredoxin‑1 in HeLa Human Cell Line. Thioredoxin-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Thioredoxin-1 Monoclonal Antibody (Catalog # MAB1970) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary ... WebGlutathione peroxidase 4 (GPX4) is a selenocysteine-containing glutathione peroxidase that is encoded by the GPX4 gene in humans and protects cellular membranes from oxidative damage.1,2 It is a monomeric protein consisting of a thioredoxin motif and a selenocysteine-glutamine-tryptophan catalytic triad that reduces lipid hydroperoxides ...
Thioredoxin 1 antibody
Did you know?
Web94 Thioredoxin 1 Primary Antibodies: Thermo Fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and … WebThioredoxin‑1 in HeLa Human Cell Line. Thioredoxin-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Thioredoxin-1 Monoclonal Antibody (Catalog # MAB19701) at 8 µg/mL for …
WebMouse anti Human thioredoxin 1 antibody recognizes thioredoxin, also known as ADF, ATL-derived factor, SASP, TRX or TXN delta 3. The protein encoded by TXN gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to … WebApr 15, 2024 · The membrane was then blocked for 25 min at RT (gentle shaking) by using the blocking buffer (LI-COR Intercept ® Blocking buffer—TBS) and then incubated with mouse anti-CoA primary antibody (1/6000 dilution in Intercept antibody dilution solution TBS-T20, REF) for 1.5 h at RT or O/N at 4 °C. The membrane was washed with 1X Tris …
WebPVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Thioredoxin Reductase 1/TRXR1 Monoclonal Antibody (Catalog # MAB7428) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin Reductase 1/TRXR1 at approximately 65 kDa (as indicated). WebThioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It is ubiquitous and found in many organisms from plants and bacteria to mammals. Multiple …
WebWestern Blot using Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (116) ( Product # MA5-29624) at 1:2,000 dilution. Lane A: Hela Whole Cell Lysate, Lane B: THP-1 Whole Cell Lysate, Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution.
WebInvitrogen Anti-Thioredoxin 1 Monoclonal (3#), Catalog # MA5-43844. Tested in Western Blot (WB) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (1 mg/mL). number in word formula in excelWebMouse anti Human thioredoxin antibody, clone 2B1 (MCA1538) used to assess thioredoxin expression levels on different cell types in culture by flow cytometry. Image caption: Cell surface expression of PDI and/or Thioredoxin. PM-1 T cells (panel A), primary human MDM (panel B) and PBLs (panel C) were isolated and cultured as described in Methods. number in word form sheetWebThioredoxin participates in many cellular processes including redox signaling, response to oxidative stress, and protein reduction (1). A potential role of thioredoxin in human … nintendo switch games on sale amazonWebWhen unfolded proteins accumulate to irremediably high levels within the endoplasmic reticulum (ER), intracellular signaling pathways called the unfolded protein response (UPR) become hyperactivated nintendo switch games mario rabbids 2 peoplenumber investment accountsWebProduct overview. Human Thioredoxin 1 ELISA Kit (ab277448) is an i n-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thioredoxin 1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human Thioredoxin 1 coated on a 96-well plate. Standards and samples are pipetted into ... number in word formatWebJan 1, 2012 · TXN1 and TXN2 primary antibodies (Santa Cruz, sc-20146 and sc-137028) were used at a dilution of 1:400 and 1:200, respectively. For TXN1 the secondary antibody was anti-rabbit-HRP (Sigma, A0545) at 1:3000. For TXN2, the secondary antibody was anti-mouse-HRP (Sigma, A8924) at 1:5000. 2.7. End-point thioredoxin enzyme activity assay nintendo switch games on sale 2021