WebNov 17, 2024 · pEALSR1 and pEALSR2 were the original vectors used for ALSV preparation. High concentrations of these vectors are prepared from colon bacteria (Escherichia coli) culture and rub-inoculated (inoculated with SiC, carborundum) onto the leaves of quinoa (Chenopodium quinoa) plants. The so-called agro-inoculation method was more … WebJun 19, 2024 · The ALSV-based vectors pEALSR1 and pEALSR2L5R5 include the complete RNA1 and RNA2 genomic sequences, respectively, under the control of an enhanced …
ALSV-Based Virus-Induced Gene Silencing in Apple Tree
WebInfectious cDNA clones of ALSV (pEALSR1 and pEALSR2L5R5) are used for construction of ALSV expressing GFP, YFP, and CFP (see note 1 and Fig. 1). 2.3 Fluorescent Proteins Plasmids pEGFP-1, pECFP-1, and pEYFP-1 (BD Biosciences, CLONTECH) are used for amplification of GFP, CFP, and YFP sequence. 2.4 Purification of cDNA Clones 1. WebMay 1, 2006 · When pGFP MP was co- bombarded with pEALSR2L5R5 and pEALSR1, multicellular GFP fluorescence was easily observed in inoculated leaf tissues (Table 1). Similarly, when a mixture of two deletion mutants of capsid proteins (pGFP Vp25 plus pGFP Vp20, pGFP Vp20 plus pGFP Vp24, pGFP Vp24 plus pGFP Vp25, p Vp25 plus Cell-to-cell … newman and goh
GR DEX system ResearchGate
WebALSV-RNA1 DNA clone (pEALSR1) (Li et al. 2004)as template, positive chain primer [T7ALSVRNA15(?) (1–32); 50-GTAATACGACTCACTATAGGGTTCGTTTT AGAGCTTGTGAGTTGTTATTACGT-30] including a T7 promoter sequence and negative chain primer [RNA2-3-polyT17(-)(6813–6782); 50-TTTTTTTTTTTTTTTTTGGA … WebPearl's Peril. 3,175,969 likes · 4,924 talking about this. Pearl's Peril is a hidden object game like no other! Get swept back to the romantic and sordid 1930’s along with our trailblazing … WebPodcast kali ini membahas tentang bagaimana interkasi kita dengan Al-Qur’an khususnya dalam kehidupan sehari-hari. Kita akan membahas kapankah waktu yang spesial antara … intramural sports auburn